CHAP ts isolate specihic peptides that bind to a specitic all of the above e of
ID: 187749 • Letter: C
Question
CHAP ts isolate specihic peptides that bind to a specitic all of the above e of the following is needed to perform yeast two hytrid Whwa vectors are neecded to express the bait and prey proteins Aweorter gene under the control of the GALA recognition DBD of a transcription factor genetically fused to the protein of interest, also called the bait e AD domain of a transcription factor genetically fused to proteins that are being screened in for interactions with bait. e. All of the above are needed what is co-immunoprecipitation used? termine if a protein-of-interest binds to a specific DNA sequence to examine protein-protein interactions in the nucleus instead of in the to to examine protein-protein interactions in the cytoplasm instead of the nucleus d. to e. none of the above allow a protein to be expressed in mammalian cell culture .What is a problem associated with immuno-based arrays? a. Proteins tha t are bound to solid supports may not be representative of intracellular conditions. The antibody may cross-react with other cellular proteins, producing a false positive c. Low concentrations of some proteins may not be able to compete for active sites compared to those that are in abundance d. Proteins that are often found in complexes may have the antibody binding site masked by the other proteins in the complex. e. All of the above are problems associated with immuno-based arrays 4. Which of the following has been extensively studied using protein interaction arrays? a. proteins in yeast that bind calmodulin or phospholipids b. proteins in yeast that bind to glutathione-S-transferase c. proteins that are able to bind to biotin and streptavidin d. proteins that are able to bind to various cofactors present in the sample e. none of the above is. Which of the following methods is the best way to analyze a metabolome? a. high-pressure liquid chromatography b. mass spectroscopy c. nuclear magnetic resonance d. thin layer chromatography e. ELISA 16. Which of the following would be the best method to identify thousands of different proteins simultaneously? a. MALDI-TOF b. traditional MS c. SILAC d. MuDPIT e. SELDI Continued)Explanation / Answer
Question 16
Answer is c SILAC( stable isotype lebelling of amino acid in cell culture) :-SILAC capicity is identify tha thousands of protein in simultaneously.it identify quantity bases.
Question 15
Answer is A - HPLC( HIGH PRESSURE LIQUID CHROMATOGRAPHY )is the best method to anylze to metabolomes. Another method also Ms,and Nmr
Question 14
Answer is D
Proteins that are able to bind to various cofactors present in the sample.
Question 13
Answer is e
All of the above are problems associated with immuno based array.
Question 12.
Answer is b
To Examine the protein-protein interaction in the nucleus instead of in the cytoplasm.
And question 11 not seen clearly to understand