Try to answer the following questions when preparing for quiz2 are the two Watso
ID: 187895 • Letter: T
Question
Try to answer the following questions when preparing for quiz2 are the two Watson-Crick base pairs in DNA double helix? Given a specific DNA sequence (eg.5-GTACGTTCGA-3'), can you derive the complementary DNA strand including proper polarity)? About PCR What does PCR stands for? What is the expected product of the PCR reaction you are going to carry out in the lab? We use PCR to amplify a fragment of the Dicer-encoding DNA, the DNA fragment should have 3x270-810 nucleotides (since the protein we want to clone has 270 amino acids). Therefore, the PCR product should be a double stranded DNA with about 810 base pairs. What ingredients will you need to put into the PCR reaction mixture? A DNA template (a plasmid containing the Dicer-encoding DNA) dNTP mix (containing dATP, dGTP, dCTP, and dTTP) providing the monomeric building blocks A forward primer (a synthetic oligonucleotide with -40 residues) A reverse primer (a synthetic oligonucleotide with ~40 residues) Taq DNA polymerase 10x reaction buffer What are the three steps in a PCR cycle? What happens in each step? Step 1: Denaturation, heat-up to 95 C to separate the DNA strands Step 2: Annealing (or hybridization), cool down to 60°C to allow primers to anneal (form complementary double helix) to the DNA template Step 3: Extention, raise temperature to 72°C (optimal for Taq DNA polymerase activity) to synthesize the new second strand of DNA by extending the primers What is the product of the digestion reaction? The cloning vector is a circular DNA plasmid. The restriction enzyme Smal makes one cut on the vector, produce a linear double stranded DNA. What are the two reasons for the use of a Bunsen Burner in the lab? 1) The flame heats the air in its immediate vicinity and causes it to rise, effectively creating an air current that takes dust particles away 2) The flame is also convenient for sterilizing equipment at the bench.Explanation / Answer
A base pair is consisting of two nucleobases which are bonded with each other by hydrogen bond. Watson Crik base pair , guanine-cytosine ( GC) and adenine-thymine (AT) helps to maintain the DNA structure.
5'-GTACGTTCGA-3'
3'-CATGCAAGCT-5' ( complimentary strand)