Pleaser answer both, the last person did not do as asked. I pay for my questions
ID: 189847 • Letter: P
Question
Pleaser answer both, the last person did not do as asked. I pay for my questions so please answer them. 12. Fluorescence microscopy of cells that are labeled with a green fluorescent antibody against microtubules (a cytoskeletal component) differs from cells expressing green fluorescent protein (GFP) tagged to beta tubulin (a component of microtubules) in all the following ways EXCEPT a. the antibody labeled cells need to be permeabilized before visualization, while the GFP cells do not. b. the microtubules could change their localization over time in the GFP cells, but not in the antibody labeled cells. c. the GFP cells do not need to be treated with fixatives, but the antibody labeled cells do. (see d on Page 2 of 3
Explanation / Answer
1. option a is incorrect as both the antibody as well as GFP needs to be permealised through the cells in order to get visualised.
2. Confocal microscopy is the correct answer.
Reason: the principle of confocal microscopy is that the excitation light is focussed on more than one discrete point in the sample specimem. At the same time fluorescence is in the excited state at the cones of illumination above and below the focus. this outfocussing of fluorescence minimised in final image by introducing physical barrier in an imaging plane before the detector. While from the focal point In-focus fluorescence passes through a small aperture in the image plane and hence detection points are co-aligned or ‘confocal. In this way we prevent blurring of the image.