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Refer to figure 2. The cell numbers refer to the total cell count in each of the

ID: 190793 • Letter: R

Question

Refer to figure 2. The cell numbers refer to the total cell count in each of the numbered small squares. If the sample is undiluted and unstained. What is the cell density. Square 1:150 Square 2: 145 Square 3: 135 Square 4: 145 e depth of each s the volume of liquid , the smal e rn. 4. Refer to the hemocytometer drawing in Figure 2. The cell numbers ref to the total cell count in each of the numbered small square is undi (Show how you figured out your answer, and include units) Square #1:150 #2: 145 #3: 135 #4: 145 #5: 150 er s. If the sample iluted and unstained, what is the cell density of the sample? Figure 2

Explanation / Answer

Average Total number of cells in 4 squares = 150 + 145 +135 + 145 + 150 / 5 = 145

Number of cells/ mm2 = 145 X 25 = 3625 .    In 1mm2 there are 25 small square are there so we should multiply that.

Number of cells/ mm3 = 3625 X 10 = 36250.   10mm is the distance between cover slip and hemocytometer.

Number of cells/ cm3 = 36250. X 103                           103 is the volume

Number of cells/ cm3 = 3.625. X 107