Materials per student group 36-48 hour milk culture of Klebsiella pneumoniae dro

Question

Materials per student group 36-48 hour milk culture of Klebsiella pneumoniae dropper bottle of India ink crystal violet (I dropper per 2 students - part of Gram stain reagents) I slide per student 1 Microscope 1. e capsule stain. Answer this question concerning what it should look like. In a capsule stain, the cry stal violet should penetrate the capsule whr'le(color) and the capsule looks but the ndra inkdoes not. As a result, the bacterial cell is purpe (color) and the background is black lare

Explanation / Answer

The fill in blanks looks perfect .

And the background mostly appears black. Capsules are fragile and can be diminished, desiccated, distorted, or destroyed by heating. A drop of serum can be used during smearing to enhance the size of the capsule and make it more easily observed in microscope

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