Please answer number 15 & first bonus question b. acultstee AerGrow in presence

Question

Please answer number 15 & first bonus question b. acultstee AerGrow in presence or absence of oxygen Require oxygen to grow and why is it 15. (2 pts) Why is thioglycollate medium appropriate for measuring aeroto important to use this medium soon after preparihion autoclaving? oxgen can a ce ine: 16.(1 pt) Descnbe the growth patterm yóu would expect to seein Clostridium rubrum in thioglycollate medium. You can use a drawing to illustrate your description. 17. (2 pts) In lab, we saw unexpected growth of a strict aerobe incubated in an anaerobic chamber. What are two different possible explanations? bad Ser BONUS (1 p) Name one G+ bacteria isolation media hiercelaieand one G that we will be using in today's lab. bacteria isolation mediaTheyedcte BONUS (1.5 pts): Name the Gram stain result color and cell morphology (must inctude both for full credit) foreachcoches olle lermited tl nec; 1( a. Staphylococcus b. Escherichia coll Proc e. Bacillus subtilisthe becu e acBoo

Explanation / Answer

15. Thioglycollate broth is designed to test the aerotolerance of bacteria.

It's constituents include nutrients for bacterial growth,

thioglycollic acid, thioglycollate, L- cysteine to reduce oxygen to water

And methylene bland am indicator for presence of oxygen

and agar which helps in retarding diffusion of oxygenn and helps in maintaining the different layers of broth containing different organisms.

This is the reason why this medium is used to measure aerotolerance.

Through autoclave get, oxygen diffuse out of the medium but when the medium sits at room temperature oxygen starts to diffuse in again that is why it is important to use this medium soon after autoclaving.

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