Regarding, DNA stacking forces, avidity increases with multivalent interactions.

Question

Regarding, DNA stacking forces, avidity increases with multivalent interactions. Let’s think of a toy model of a dimeric receptor binding with a divalent ligand. The KDs for binding between a two-armed compound LL1 (this is the ligand) and individual receptors R1 and R2 are:

R1 … 7.9 nM             R2 … 50.1 nM

Now think of a protein R1-R2, which is a protein comprised of both R1 and R2. At a temperature 300K, if there is no loss of energy/entropy to induce conformational changes of the protein and ligand during the binding, what is the expected KD between LL1 and R1-R2 and corresponding deltaG?

Explanation / Answer

Acc to thumb rule , at 298 k a delta - delta g difference of 1.4 kcal/mol corresponds to a 10 fold difference in kds . ......... Kd = (L) (R)/ (LR)

DeltaG = RTlnkd

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