Please only check the answer for part C if right or no Please please be accurate
ID: 192332 • Letter: P
Question
Please only check the answer for part C if right or no Please please be accurate and tel me if it is right if not then please correct Thank you so much! 2. Your friend shows you her data. She has been analyzing a protein (CB353) which is 576 amino acids long to determine which parts of the protein are responsible for its localization. She has created various deletion mutations in her protein and examined its localization, under normal conditions and also in the presence of leptomycin B, an inhibitor of the nuclear export machinery. Her data are depicted below. 9 marks total. localization in the presence of eptomycin B localzation acids nucleus cytoplasm nucleus cytoplasm 1-576 115-576 240-576 358-576 240-357 115-357 1-114 115-239 From her data, answer the following questions: A. Which amino acids contain the nuclear localization signal (NLS)? Briefly explain. (3 marks) B. Which amino acids contain the nuclear export signal (NES)? Briefly explain. (3 marks) NES is present in 1-239. 1-84 and 15-357 because it shows localization in nucleus and cytoplasm under nomal conditions C. Describe a microscopic technique and procedure that your friend used to generate this data? (3 marks)Explanation / Answer
Yes, you are right. Protein localization can be determined using fluorescence microscopy. The protein whose localization needs to be determined is tagged with fusion proteins and made to overexpress. These fusion proteins can then be detected using antibodies or if they are fluorescent, then optical imaging procedures can be used.