ANp63, involucrin and and b). What kind of . A western blot was performed to det
ID: 192579 • Letter: A
Question
ANp63, involucrin and and b). What kind of . A western blot was performed to detect expression ona and b). Keratin 10 in keratinocytes (middle panels of Figure 1a a molecule is the "probe" in a western blot? RNA DNA e protein antibody What kind of molecule is the target of the probe in a western blot? RNA DNA e protein antibody According to the data, expression of which of the following increases in mouse keratinocytes as they undergo differentiation?- Choose two answers. note time 0 is undifferentiated and time 7 is mature, fully differentiated cells. " miR-203 keratin 10 * involucrin U2 snRNA actin . What type of experiments are presented in this figure? Choose any that apply Find it .Lose it e Move it .Which of the following is a conclusion that can be drawn from the data presented in this figure? Select any that apply. Choose two answers. Involucrin and miR-203 co-localize to the same compartment in mouse keratinocytes Expression of miR-203 increases during differentiation of mouse keratinocytes. miR-203 decreases the expression of Np63 miR203 induces differentiation of keratinocytes Keratin 10 expression increases during human keratinocyte differentiation Actin does not play a role in keratinocyte differentiation Np63 is strongly expressed in the basal layer of the skin · .What is the major difference between the kind of information that can be obtained from a western blot vs. immunofluorescence techniques?Explanation / Answer
Probe :It a molecule of known nature which is labeled either with radioactivity or a fluorescence and is used to identify either nucleic acids (DNA) or proteins. Western blotting is a technique for the separation of protein through gel electrophoresis. In western blotting a probe is therefore an antibody that binds specific target protein.
2. Since, Western blotting is used for the separation of protein, therefore, protein is the target molecule of the probe (antibody).
Question numbers 3, 4 and 5 can't be answered because data and figures are not given, through which answers could be given.
Difference between western blot and immunoflourescence :
Western blotting is a technique also called as immuno protein blot. It is used for the separation and identification of specific protein from a cell or tissue extract. Here, proteins are separated through gel electrophoresis and then hybridized with antibody (probe).
On the other hand, immunoflourescence is a technique which is used for microscopic observation of protein, their location and distribution within the cells. In this technique a fluorescent antibody is used that reacts with the epitope of antigen within the cells and flouresces when observed under fluorescent microscope.