DESIGN PCR PRIMER FOR THE FOLLOWING SEQUENCE 50% G/C, AT LEAST 20 nt in leght Ca
ID: 192740 • Letter: D
Question
DESIGN PCR PRIMER FOR THE FOLLOWING SEQUENCE 50% G/C, AT LEAST 20 nt in leght
Calculate each primer Tm .
1 ATACACTTAAACTTCTTTGCAAAACTTTCATCAAAATATTATATTTAGTATGAATTCCGC
Start codon
61 TCGGTTCTACGCTCAATACAGCATGTCCCGTCTCGTAAATAGTGTGACTACTTTTAAGAG
121 TAGTGGCGAATCTTGCAAGTTGCATCGAATTTACAAAATTAGTTTTCCTTCGGGTCAAAA
181 GTATATTGGACAAACCGTGAAAATGCCCAATGAACGCCTCAAGGAACACCAACGAGAATC
241 ATCCAAGTGTCATAGGCTAAAACACGCTCTCCGAACAAATCCTGATTTCAAATTAGACAC
301 GGTCGCGATTGTAGGACCTCACCAGGTTCATGCTGTAGAAAAAATTGCCATTGCTCTCGA
361 AGATTCAACAGGTCCAAACGGCCTTAACATAACCATCGGGGGTGCTGGAGTGAAGAAGCC
421 AGATGAAAGATACGAAACTTACAAGAAGCACGTTAGTGATATATATCATCGTATGAAGAG
481 AGGATCTTTCGTATCATACGACGTTTTGTTCATGAAAAATGATACTATGCTATCCGAAGA
541 AGAATTTCAAGCCATCAGACGGCTTTTGTAAAATCACCGGGATTCTAATAATTTCACACG
601 TTCTTCTAGAGTTTCAAGAGA
Explanation / Answer
Primer pair 1
Primer pair 2
Primer pair 3
Primer pair 4
Primer pair 5
Primer pair 6
Primer pair 7
Primer pair 8
Primer pair 9
Primer pair 10
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity Forward primer ATACAGCATGTCCCGTCTCG Plus 20 84 103 59.61 55.00 4.00 2.00 Reverse primer TTGGTGTTCCTTGAGGCGTT Minus 20 240 221 60.11 50.00 4.00 0.00 Product length 157