I. Consider the following RNA, transcribed from the complementary DNA. The RNA c

Question

I. Consider the following RNA, transcribed from the complementary DNA. The RNA contains introns and exons. The exons are in uppercase, introns are in lowercase. After splicing, what will the sequence of the RNA be? ATCACAAACTAGATGGTACgtgacagatacagtagtagtgaagtagCAGATAATATTAGG 2. Explain the method of GFP fusion genes in your own words (see video 5b!) 3. For the following methods, do we study expression at the RNA or protein level? In situ hybridization: Western blot 4. You are studying a species of lizard that has no external genitalia or clear differences between sexes, but which is known to have X/Y sex determination. Would you guess that the individuals below are male or female? “ ^ | Individual A? Individual B? 5. Please label the appropriate arrows with 'transcription', 'translation', and splicing. DNA initial RNA spliced RNA protein

Explanation / Answer

RNA sequence will be -ATCACAAACTAGATGGTACCAGATAATATTAGG ( Introns will be spliced out)

3. By Insitu hybridization we can map the chromosome from where the m-RNA expression takes place.

By western blot we can identify or separate a protein.

5. DNA ---> Initial RNA ( Transcription)

Initial RNA------> spliced RNA ( Splicing)

Spliced RNA ----------> Protein (translation)

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