Please help and please show all work. I am not entirely sure what to do with thi
ID: 195891 • Letter: P
Question
Please help and please show all work. I am not entirely sure what to do with this question.
A generalized transducing phage is used to transduce an abcde recipient strain of E. coli with an atb c d e donor. The recipient culture is plated on various media indicated in the second column of the table below, with the results shown in the third column. (Note that"a" indicates a requirement for A as a nutrient, etc.) Experiment Number to minimal mediumabsence (-) of colonies 17. Compounds added Presence +) or CDE BDE BCE BCD ADE ACE ACD ABE ABD ABC 4 10 a. What genotype is being selected for in each experiment above? b. Which of the above markers can be cotransduced? c. What can you conclude about the linkage and order of the five genes?Explanation / Answer
a) The genotypes a+b+c+d+e+
b) If a compound is not added and growth occurs, the E. coli has received the genes for it by transduction. Therefore, the BCE culture must have received a+ and d+. The BCD culture received a+ and e+. The ABD culture received c+ and e+.
c) The order is thus d a e c. The b is never cotransduced, and therefore, it maintained distant from this group of genes.