Remaining Time: 54 minutes, 56 seconds. Question Completion Status: The enzyme a
ID: 196383 • Letter: R
Question
Remaining Time: 54 minutes, 56 seconds. Question Completion Status: The enzyme adds three nucleotides of DNA in the ONA polymerase III,3'5. O DNA polymerase I, 3-5 O DNA polymerase II, 5-3 O DNA polymerase 1, 5 3 O None of the above direction when DNA is being semi-conservatively replicated QUESTION 30 Gene probes can be labeled for detection with O enzymes tuorescent dyes O radioisotopes O Al of the choices are correct O None of the choices are correct QUESTION 31 Both DNA sequencing and polymerase chan reactions require special O RNA promoters Sorescent nucleotides O primers O thermophilic polymerases O endonucleases to ntiate synthesis of a new DNA molecule QUESTION 32 Some genes in bacteria are repressed by antisense RNA that binds to them O True O Fase QUESTION 33 Transformation and transfection are methods used to introduce DNA into host cells O True O False QUESTION 34 The Southern Blot echnique detects O DNA O RNA O proteins O recombinant DNA O specific genetic marker sequence on genesExplanation / Answer
29. Option 3rd is correct. DNA replication occurs in 5' to 3' direction and the enzyme used for addition of nucleotides is polymerase III.
30. Option 4th that is all of the choices are correct. Enzymes, fluorescent dye and radioisotobes all are used for labelling of gene probe for detection purpose.
31. Option 3rd that is primers is correct. Primers initiate the process and letter on DNA polymerase add nucleotides. These primers are removed later on.
32. True, antisense RNA is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) that hybridize with it and thereby blocks its translation into protein.
33. True
34. Last option is the correct answer. Southern blotting technique is used to detect a specific DNA sequence.