Secure Week 4 Homework () 6of12 Computational Blochemistry: Identification and I
ID: 196737 • Letter: S
Question
Secure Week 4 Homework () 6of12 Computational Blochemistry: Identification and Investigation of an Unknown Protein You ane a computational biachemist participating in this inbernational effort Your project is to idenily and investigate funcion of the unknown human gone labeled FABPS Neither the structure of the protein for which this gene codes, nor its function, is known The sequence of the gene was submitted to the sequence database GenBank Genark ithe NIH genetic sequence database, an antated coldon of al ptldy avalable DNA Genstic material provides a wealth of informasion that can provide insight into the structure and function of the proteitn it codes during translation Numerous computational methods have been developed to analyze, compare contrast and characterice gene and protein sequences In this case study, you will use a Basic Local Alignment Search Tool (BLAST) sequence alignment and analysis, and information obtained from the Protein Data Bank (PDB) to lidentify and investigate the sequence of the unknown gene and the resulting protein it codes ORIGIN: Gene sequence 1 TACOGAAAGT GGCCGTTCAA GCTCTACCTC TCACICIICT TAATACTACT CAAGTACTTC 61 GAGGAACCCT AGAGTOGCT ACATTAGCTT TTOCGGGCGT TGAAGTICTA GCAGTGOCTO 121 CACGTCGTCC TACCCGTOCT GAAGTGAACC AGGGTOGTGA TGAGGCCOCC GGTGTGGTAC 241 TCCGGTGAC ACGTCTACCT cccGcccTTC GACCACCACT TAAAGGGGTT GATAGTGGTC 301 TGGAGTCTCT AGCACCCACT GTTCGACCAC CTCCAGAGGT GGTGCCTCC GCACTGGATA The Human Genome Project is an international sclentific effort writh the goal of determining the sequence of nucleotide base pairs that make up human DNA and of identifying and mapping all of the geenes of the human genome rains h* world's largest collaborative biological project. In the US, the project launched in 1990 and was declared complete in 2003 Funding came from the US povemment through the National Instihutes of Health (NIH) National Science Foundation (NSF) as well as numerous oher groups from around the workd Part A. Transcrption of DNA Transcribe first 60 nucleotides of DNA into its corresponding mRNA sequence Enter the cornesponding mRNA sequence mRNA sequence The next stop in the study of biological systems was to translate the predicted gones into protein sequences and stedy the available proteome, the entire set of probeins produiced by an organism This new area of blochemistry was named proteomes its goal is a large-scale study of proteins, their structure and function Numerous labs around the world suboequently turned their efforts to clessifying proteins of unknown function by using sequence analysis and similar methods developed by computasional blochemlsts Subm Part B hs qvestion will be shoun atter yo complete previous questong) pe here to searchExplanation / Answer
to transcribe DNA to RNA=>ATGC sequence is changed to UACG so fpr 1-60 in question
AUGGCUUUCA CCGGCAAGUU CGAGAUGGAG AGUGAGAAGA AUUAUGAUGA GUUCAUGAAG
as mrna is always made up of three codons:
AUG GCU UUC ACC GGC AAG UUC GAG AUG GAG AGU GAG AAG AAU UAU GAU GAG UUC AUG AAG