Please include a detailed explanation for both parts of the problem so I can und
ID: 197146 • Letter: P
Question
Please include a detailed explanation for both parts of the problem so I can understand how it was done and what is occurring. ThanksAnalysis After 2-3 days of growth, you should begin to see the resulting anertype of your diploid col J phenotype of your diploid colonies on both the YED and MV plates. If e cells from the two haploid strains were not in equal proportion or . You mixed thoroughly, there may be pink/red areas in an otherwise cream-colored colony should score these diploids as cream-colored. 1. Record your results in the grids below. (Use R = Red; C Cream for YED. Use +--Growth:--No Growth for MV) 0 YED MV 2 3
Explanation / Answer
YED
1 0a0, 0a1, 0a2, 0a3 – CC – complementary group
2 1a0, 1a2 – CC - complementary group
3 1a1, 1a3 –RR - mutants
4 2a0, 2a1 – CC - complementary group
5 2a2, 2a3 – RR - mutants
6 3a0, 3a1 – CC- complementary group
7 3a2, 3a3 – RR - mutants
MV
1 0a0, 0a1, 0a2, 0a3 – (++) - complementary group
2 1a0, 1a2 – (++)-complementary group
3 1a1, 1a3 – (--) mutants
4 2a0, 2a1 – (++)-complementary group
5 2a2, 2a3 – (--) mutants
6 3a0, 3a1 – (--) mutants
7 3a2, 3a3 – (++)-complementary group
There are 20 complementary groups, 10 from each plate type.
1a1, 1a3, 2a2, 2a3, 3a2, 3a3 are mutants in YED group
1a1, 1a3, 2a2, 2a3, 3a0, 3a1 are mutants in MV group
It is mentioned in the question that if the two haploid strains were not mixed in equal proportions, they are red or pink in YED and ‘–‘ in the case of mutants.
Hence, it does not mean that it is dominant or recessive.
Therefore, we cannot conclude that mutants are either dominant or recessive.