Consider the mitochondrial electron transport chain that utilizes NADH a. Write
ID: 197815 • Letter: C
Question
Consider the mitochondrial electron transport chain that utilizes NADH
a. Write a net balanced equation for the reaction run by Complex I. Include the protons pumped from the inside to the outside of the matrix.
b. If you assume that the membrane potential is 200 mV and the pH gradient is 0.5, how much energy is stored by Complex I as a membrane proton motive force per mole of NADH consumed?
c. Now write down the two half reactions the make up the reaction from part (a) (ignoring the protons pumped).
d. Using the values for E0 in your book for these reactions, compare the total energy of the reaction you wrote down in (a) to that stored as the proton motive force.
Please show work for a-d thanks.
spascete sideComplex space (P side) H+ Ht H+ H+ QH2 2e Matrix arm N-2 Membrane arm 2H* Series of Fe-S centers Matrix (N side) FMNe 2e NADH+H+ NAD+Explanation / Answer
Answer 1.
NADH+ H+ + Q + 4H+ (MATRIX SIDE) NAD+ + QH2 + 4H+ (P SIDE)
Answer 2. Proton motive force ,
where is the membrane potential (in mV) with negative sign, 2.303RT/F = 60 mV at 300 K (27 oC)
by putting all these values in above equation the answer is = -230 mV
Answer 3. NADH + H+ NAD+ + 2H+ + 2e-
Q + 2H+ + 2e- QH2