In order to obtain higher spatial resolution in microscopy, one can use an oil-i
ID: 1981349 • Letter: I
Question
In order to obtain higher spatial resolution in microscopy, one can use an oil-immersion objective. A drop of oil is placed on the sample, and the objective is brought into contact with the oil. However, the eyepiece-side of the objective is still in air. Suppose you have an objective consisting of a lens that is convex on both sides and for which both sides have a radius of curvature of magnitude 0.99 mm. Suppose that the lens is made with an index of refraction of nlens=1.550 and that the oil has an index of refraction of noil=1.470. What is the effective focal length of the objective when the sample-side is in oil (that is, how far must the lens be from the sample for the image created by the objective to form at infinity)?Explanation / Answer
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. hence our new focal lngth =f=(R/2)(n1/n2)=0.521mm R=radius of curvature n1=lens refractive index n2-.........of oil