Which of the following are TRUE statements concerning HAT selection? O HAT selec

Question

Which of the following are TRUE statements concerning HAT selection? O HAT selection requires the fusion of helper T cells with myoloma cells. DMyoloma cells are killed as they are mortal. DYou isolate only Hybridoma clones that recognize your specific antigen from HAT selecti D Myoloma cells die as they cannot synthesize new nucleotides. DHAT selection requires the fusion of plasma cells with myoloma cells. DHAT medium selection is used to generate a polyclonal antibody. HAT medium selection is used to generate a monoclonal antibody.

Explanation / Answer

Ans. HAT (hypoxanthine aminopterin thymidine ) is used to generate monoclonal antibodies.

It is used for hybridoma selection I.e hybridoma means fused cell (myeloma+normal cell.) only hybridoma cells survive due to presence of an enzyme HGPRT. Also myeloma cells die as they can't synthesize nucleotide de no one.

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