Study Guide- Lecture Exam 2-Protected View-Saved to this PC View Tell me what ca
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Study Guide- Lecture Exam 2-Protected View-Saved to this PC View Tell me what can contain viruses. Unless you need to edit, it's safer to stay in Protected View. Enable Editing nanunmng, ing, resolution. lenses. Suayng ourgaTUSS 3.03 Describe the basic plan of an optical microscope, and differentiate between magnification and 3.04 Explain how the images are formed, along with the role of light and the different powers of 3.05 Indicate how the resolving power is determined and how resolution affects image visibility 3.06 Differentiate between the major types of optical microscope, their illumination sources, image appearance, and uses. Describe the operating features of electron microscopes and how they differ from optical microscopes in illumination source, magnification, resolution, and image appearance 3.08 Differentiate between transmission and 3.09 Explain the basic differences between fresh and fixed preparations for microscopy scanning electron microscopes in image formation and are used 3.10 Define dyes and describe the basic chemistry behind the process of staining 3.11 Differentiate between negative and positive staining, giving examples 3.12 Distinguish between simple, differential, and structural stains, including their applications 3.13 Describe the process of Gram staining and how its results can aid the identification process. 3.14 Define inoculation, media, and culture, and describe sampling methods and instruments, and what events must be controlled. 3.15 Describe three basic techniques for isolation, including tools, media, incubation, and outcome 3.16 Explain what an isolated colony is and indicate how it forms 3.17 Differentiate between a pure culture, subcultare, mixed culture, and contaminated culture. Define a contaminant. 3.18 What kinds of data are collected during information gathering? 3.19 Describe some of the processes involved in identifying microbes from samples 3.20 Explain the importance of media for culturing microbes in the laboratory 321 Name the three general categories of media, based on their inherent properties and uses 3.22 Compare and contrast liquid, solid, and semisolid media, giving examples 3.23 Analyze chemically defined and complex media, describing their basic differences and content 3.24 Describe functional media, list several different categories, and explain what characterizes each type of functional media content and purposes Describe live media and the circumstances that r 3.25 Identify the qualities of enriched, selective, and differential media, use examples to explain their 3.26 Explain what it means when microorganisms are not culturable 3.27 equare itExplanation / Answer
3.03) Optical microscope is the simple light microscope that magnifies the image of a specimen. It uses an objective lens and magnifies the image further using an eyepiece that allows the user to observe it by the naked eye. The objective lens creates a magnified inverted image and the eye piece magnifies the image further and creates a virtual erect image. Magnification is the degree to which an object is magnified (made bigger) and resolution is the degree to which two closely spaced points are can be distinguished as separate entities.
3.05) Resolution is determined by the ability to differentiate two closely placed points in an object. The general formula for calculating resolving power is
= 0.61 * (/N.A)
This equation is known as Reyleigh formula where
= wavelength and N.A = Numerical aperture of the objective lens