The diagram below represents an analysis of lymphocytes treated with two distinc

Question

The diagram below represents an analysis of lymphocytes treated with two distinct monoclonal antibodies that were tagged with different fluorescent tags. The dots in the histogram below represent individual cells and the intensity of the dots represents the relative cell concentration. The color of the dots in each square is not important. color is simply used for the purposes of clarity. One of the monoclonal antibodies used in the experiment reacted with the CD3 marker whereas the other monoclonal antibody reacted with the CD19 marker. Using the diagram below select the correct answer from the ones given below: 1. Dark blue dots (panel A) represent B cells, yellow dots (panel B) Represent T cells, and the light blue dots (panel C) represent neither T nor B cells. 2. Dark blue dots (panel A) represent B cells, yellow dots (panel B) represent neither T or B cells, and the light blue dots (panel C) 2 C represent T cells Increasing CD19 Fluorescence 3. Dark blue dots (panel A) represent T cells, yellow dots (panel B) represent neither T nor B cells, and the light blue dots (panel C) represent B cells 4. Dark blue dots (panel A) represent T cells, yellow dots (panel B) represent B cells, and the light blue dots (panel C) represents neither T nor B cells

Explanation / Answer

The correct answer based on the diagram is 3) dark blue dots represent t cells, yellow dots represent neither T or B cells, and the light blue dots represent B cells.

The answer is based on the fact that CD3 marker is observed on all stages of T cells while CD19 is observed on b cells.

so max CD19 fluorescence is seen on C or b cells while max CD3 fluorescence is seen on A or T cells.

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