Pompe disease is caused by a deficiency in the enzyme alpha-glucosidase (GAA). A
ID: 201484 • Letter: P
Question
Pompe disease is caused by a deficiency in the enzyme alpha-glucosidase (GAA). A biotech company would like to synthesize GAA in sheep so that the protein can be purified from their milk. Casein is a protein that is normally produced in sheep’s milk. The sheep casein gene is 5KB and the promoter for the sheep casein gene is 1KB. The human GAA gene is 10KB. The promoter for the human GAA gene is 2KB. What would the size of the entire transgene be that is only expressed in the sheep’s milk?
a) 3 KB b) 15 KB c) 12 KB d) 6 KB e) 11 KB f) 7 KBExplanation / Answer
I believe the correct answer to be E) 11 KB because gene for casein is not needed but its promoter is needed to make sure that the expression occurs only in the sheep s milk. So, 10kb gene + 1kb promoter region from casein gene would result in a transgene of 11 KB.
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