Match the process with the best method Below are a variety of technical methods
ID: 203256 • Letter: M
Question
Match the process with the best method
Below are a variety of technical methods and molecular events and processes Connect each process with the best method for its analysis or detection (refer back to the journal club presentations). Some methods will be used more than once. B. Detection of fragmentation of A ELISA genomic DNA B. Gel Electrophoresis C. Fluorescence Microscopy C-Detection of chromatin . condensation in cells using Hoechst dye D. Western blots/Immunocytochemistry C. in vitro detection and quantification of caspase activity D. Detection of proteolytic cleavage products (such as cleaved PARP) | C. | Differentiation of apoptotic from necrotic cells by propidium iodide staining D Detection of Bcl-2 proteinExplanation / Answer
Answer=
1]Detection of fragmentation of Genomic DNA = B]Gel electrophoresis
2] Detection of chromatin condensation in cells using Hoechst dye= D]Western blot/Immunocytochemistry
3]in vitro detection and quantification of caspase activity= D]Western blot/Immunocytochemistry
4]Detection of proteolytic cleavage products (such as cleaved PARP)= D]Western blot/Immunocytochemistry
5]Differentiation of apoptotic from necrotic cells by propidium iodide staining=C]fluorescence microscopy
6]Detection of Bcl-2 protein= D]Western blot/Immunocytochemistry