3. A lab supervisor tells a student to dissolve 6 mg of Protein X in a total of
ID: 204444 • Letter: 3
Question
3. A lab supervisor tells a student to dissolve 6 mg of Protein X in a total of 15 ml buffer. The student becomes distracted by a text message and when she prepares the solution, she accidently dissolves 8 mg in 16 ml. a) Is the concentration of the solution the student prepared higher or lower than the required concentration? Show the calculation that allowed you to arrive at this answer. The student asks the supervisor if she can start over. However, the student is told that Protein X is very expensive and she cannot start over. Instead, the student needs to adjust the volume to reach the required concentration. How does she do this? Again, show your workings to support your answer. b)Explanation / Answer
=0.4mg/ml.
=0.5mg/ml.
So, the student prepared a solution with higher concentration than required.