Please Answer ALL questions. Thanks! 4) A 2008 study looked at the use of transg

Question

Please Answer ALL questions. Thanks!

4) A 2008 study looked at the use of transgeneic Aspen (Populus tremula) in 2,4,6-trinitrotoluene (TNT) contaminated soils. Aspens have deep roots, are fast growing, and have a high transpiration rate, which makes them useful in phytoremediation (using plants for remediation). Populus tremula was transformed with the bacterial nitroreductase gene pnrA. The following figure was in the article wt Figure 1 Growth of aspen in soil contaminated with different mg TNT/kg 2concentrations of TNT after 56 days. 250 +50 Photographs of (A) wild-type and (B) 70 pnrA expressing aspen. (C) Shoot dry 1000 weight and lengths of (D) wild type and (E) pnrA expressing aspen in soil after 56 days. For all graphs standard error bars are given at a confidence interval ofp

Explanation / Answer

Question 1)

C measures dry weight of plants against increasing TNT concentrations, we observe the dry weight of pnrA infected specimen is more than the wildtype. we can infer that the plant with the pnrA gene is able to tolerate and grow in TNT concentrated soils opposed to wild type's inability to do so

D and E measures vertical growth of wildtype and pnrA infected plants against TNT concentrations and we find a significant growth rate of pnrA infected plants even in TNT concentrations. With all these data available we can conclude that the pnrA gene fosters TNT tolerance and possible assimilation in plants infected.

Question 2

The gene needs to be excised from the bacteria using Restriction Enzymes and ligated into Agrobacterium tumefaciens. This virus can be used to infect plants with the genes. Plants will make copies of the gene and transcribe and translate the gene into protein products capable of TNT assimilation resulting into the phenotype.

Question 3

We need to be able to produce saplings and test them for the ability demonstrated by the parent plant. Only then the product can be commercially used.

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