Colar of Mcobactenium imegmars gher successful acid fast experimentKE 46 Color o
ID: 207379 • Letter: C
Question
Colar of Mcobactenium imegmars gher successful acid fast experimentKE 46 Color of Pieudomonas aernginosa alter succesaful Gram stain experiment Ps Numbers 47 50. Match the staining procedures described below as A Ziehl Neelsen method of acid fast staining technique B. Negative staining technique C. Capsule staining technique D. Schaeffer Pulton spore staining technique E. Gram staining technique 47 Makes use of an acidic dye. Cells "re observed as transparent in·colored 48. Carbol fuchsin as 49. Malachite green background Nag Stmu in serves as primary stain, heat as mordant and methylene blue as counter stain 2nt Neak serves as primary stain, heat as mordant and safranin as counter stain Echafer Ro t staining technique involves both negative and direct staining procedures capsota 51. Neisseria gonorrhea can be described as while Treponema palladium is a A diplococci; rickettsia B. streptococci, spirochete C streptococci, rickettsia D. spirillium, streptococci E diplococci, spirochete Questions 52-53 A urine specimen was diluted 1:100. A calibrated sterile loop was then used to deliver 0.01 mi of the diluted specimen onto nutrient agar to produce evenly distributed colonies after incubation. The colony count on the agar plate was 80. Answer the following questions. 52 The number of colony forming units (CFU) per mL of the onginal sample can be estimated tobe A. 160,000 B. 80,000 C. 8,000 ol 10,000 800,000 53. The result from the urine sample above represents non significant bacteriuria significant bacteriuria significant bacteremia D, non siguificant bacteremia of Sodium thioglycollate in media that can support anaerobic growth is to Act as an earyme to use up oxygen in the medium Act as an indicator to detect presence of oxygen in the mediunm 54, C Ad to lower the oxidation reduction peteptial thereby reduce oxygen in the mediym D. lialp in idasitying the hactéñia antExplanation / Answer
Answer 45: The acid fast staining of Mycobacterium smegmatis is PINK, because it can sustain the carbol fuschin dye and thus appears pink when observed.
Answer 46: Colour of Pseudomonas aeruginosa is PINK after Gram staining because this is a Gram negative bacteria. Gram negative bacteria have a lipopolysaccharide layer in their cell membrane, which gets dissolved after addition of alcool in Gram staining process. So, counter dye, safranin is taken up by the cells and Cells appear PINK , when observed under the microscope.
Answer 47-50:
A: Ziehl Neelson stain: 48. Carbol fuschin is used as primary stain and methylene blue as counter stain( because this is the staining procedure for acid fast bacteria.)
B: Negative staining technique: Cells appear transparent in a colored background (in this bacterial cells are not stained. acid fast dye is used to stain the background).
C:Capsule staining: The staining involves both negative and direct staining processes.
D: Schaeffer- Fulton stain: Malachite green is used as primary stain because this method is used to stain endospores.
Answer 51:
Neisseria gonorrhoeae is a diplococci while Treponema pallidum is a spirochete.