Which FACS plot represents a population of S. pombe that have been transferred i
ID: 210489 • Letter: W
Question
Which FACS plot represents a population of S. pombe that have been transferred into a minimal liquid media ( i.e. media containing no growth factors, with barely enough essential amino acids and glucose to permit cell survival) then incubated for 6 hours prior to analysis?
(Note, assume that FACS plot #1 shows analysis of a population of S. pombe replicating asynchronously (i.e. each cell within a population is at a random stage of the cell cycle) in culture. In the rich media assume the yeast have a cell cycle time of 100 min, with G1=50 min; S = 25 min; G2 = 15 min and M = 10 min).
#1 #2 #3 cells in G phase #4 #5 #6 cells in Gz and Mphases cells in Sphase relative amount of DNA per cell arbitrary units)Explanation / Answer
Answer - 3
Due to no growth factors the cells undergoes cell cycle arrest. Cells are arrest in G1 phase, which fails the G1 checkpoint due to limited nutrients or extracellular signaling.