Please answer both parts, I will leave thumbs up! You are studying a new species
ID: 210909 • Letter: P
Question
Please answer both parts, I will leave thumbs up!
You are studying a new species of microorganism that was discovered at the bottom of the ocean and appears to have been isolated from other species for millions of years. You begin analyzing the species and you realize that it does still use the same four bases of DNA and RNA that are found in the rest of living organisms - you know this because you sequenced the DNA that encodes several proteins. However, when you begin to analyze its proteins, you realize that none of the proteins contain the amino acid Alanine, only the 19 other common amino acids. Assume that the organism uses the standard triplet code in explaining your answers.
A. What are two possible explanations for why none of the proteins have the amino acid Alanine present?
B. How would you determine whether either of these two possibilities are likely to be correct? If possible, please describe the experiment and include the experimental set-up used by Nirenberg and Leder if possible to test one of your hypotheses. Assume you can isolate the ribosomes and charged tRNAs from this species and specifically radiolabel each amino acid.
Explanation / Answer
Answer
The given image shows the triplet code of which is recognised by tRNA contains complementary sequence at one end and another end binds with amino acid recognise this sequence.
A) First possible region for the absent of alanine amino acid is defect in the gene responsible for alatRNA recognised the alanine specific triplet code given in the above image.
The second possible region is defect in aminoacyl tRNA synthase binds incorrect amino acid to ala-tRNA.