The scientist then performs an experiment mating the two haploid yeast together
ID: 211448 • Letter: T
Question
The scientist then performs an experiment mating the two haploid yeast together to determine which mutation is in the DNA-binding protein and which is in the DNA-binding site. The experimental setup is listed below, wherein a minus indicates a loss-of-function mutation, and a plus indicates the wild-type allele. 2 huh1- huh2 nop1" nop2 huh1' huh2 nop1' nop2 huh1' huh2 nop1 nop2 huh1 huh2* nop1* nop2 Continued below.. How will nop1 and nop2 be expressed if huh1 encodes the DNA-binding protein and huh2 is a mutation in the binding site? How will nop1 and nop2 be expressed if huh2 encodes the DNA-binding protein and huh1 is a mutantion in the binding site? Experiment 2 Experiment 1 Experiment 2 nop2 Select answer noExplanation / Answer
huh- means the binding protein aha mutation and not working. in the same way when huh+ is a binding site where DNA binding product will bind is it is acitive.
in 1st picture
haploid 1
1. huh1 - hence dna binding protein is inactive and binding site huh2 active, nop 1- will not express and nop 2- will expres.
2. huh1 - hence dna binding protein is active and binding site huh2 inactive, nop 1- will not express and nop 2- will expres.
haploid 2
1. huh1 dna binding protein and huh2 dna binding site both are active, nop 1- will express and nop 2- will not expres.
1. huh1 dna binding protein and huh2 dna binding site both are active, nop 1- will express and nop 2- will not expres.