Can someone explain why the answer is #B? I was really confused. 9100 Chrome @ h

Question

Can someone explain why the answer is #B? I was really confused.

9100 Chrome @ https:/ canvas u davis edu courses 184926/file folder Lecture%20sides%20and%20Study%20Guide Lecture A groups of scie x-( ) X Y G mrna transcriptix vtelomerase- x 2022%20Th. > ,3 Question Q22.3. This medium/hard difficulty question is related to Learning goal L90 PEQ 19.3 A groups of scientists are interested in mapping the location of the bacterial promoter on the DNA. Using PCR, they amplity four DNA fragments (A, B, C, D): Fragment A is the longest and contains all four regions (#1, #2, #3, #4). Fragment B contains three regions (#2. #3, #4), Fragment C contains two regions (#3, #4). Fragment D contains one region (#4). The fragments were then place upstream of another DNA fragment, which encodes the enzyme galactosidase. This allows the scientists to use B -galactosidase production as a way to measure promoter strength. The data are presented below. | #1 | #2 |#3 | #4 15,000 10,000 5,000 A B C D A reasonable interpretation of these data are that the promoter is located in a. Region #1 b, Region #2 C. Region #3 d. Region #4 x MacBook 5 6 8

Explanation / Answer

Answer: Option B is correct.

Explanation:

There is no change in gene expression level between construct A and construct B. This shows that region 1 is not essential for gene expression.

Constructs C and D do not show gene expression (or reduced expression). Both these constructs lack region 2.

So, it is clear that regulatory elements required for proper gene expression are located in the region 2.

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