I just need a general explanation of the results for figure A & B (highleted por
ID: 213620 • Letter: I
Question
I just need a general explanation of the results for figure A & B (highleted portions of this mice experiment). Any feedback helps. Please explain figure A & B. A — used western blot & B—used real time RT-PCR.... A P18 >5M Ctrl. S100 65 kD 100A9 NF-kB 65 ND pStat-3 IL-1F8 S100A8 S100A9 ICAM-1 P38 38 kD P18 Epidermis 1000 228 bp S100A8 S100A9 IL-1F8 100 280 bp 10- 156 bp 165 bp T Cell 420 bp 0.1- IL-1 IL-1F8 S100A8 S100A9 Ctrl" Keratin 14 252 bp K5-R1M2 RPS29 165 bp Figure o. Progressive inflammation in the skin of K5-R1/R2 mice through production of cytokines by activated keratinocytes and yo T cells. sate mga tre Land i 5, R e at the ogedp18 and 5 M were analyzed by Western O ngw the levels of pho her late andlet ustng Tealtime RT.PCR. Gapdh mRNA was used for normalization. Results are shown in the table. -, not detectable RNAs from isolated dermis and epidermis of mice and l were analyzed +, weak expression; ++, moderate expression: +++, strong expression. IC RNAs from the epidermis of K5-R1/R2 mice and littermate controls at P18 were anolyzed for expressign of inflammatory morkers usExplanation / Answer
A. Western blotting data.
Comparison has been done between control and K5-R1/R2 mice at the age of P18 and 5 months. The activation of proteins has been looked at by the levels of phophorylated forms of Stat3, p38, NF kappa B. Phorphorylation of NF kappa B increases in mice at 5 month as compared to the controls. In p18, the activation od NF kappa B does not occur. The Phospho forms are normalised. TO the non Phospho forms of the proteins. Similarly, stat3 Phospho levels increase when mice are at 5 months age. And also, tje levels of p38 increase in P18 and 5 months old mice in K5-R1/R2 when compared with the controls.
B. Real time data has been shown which deals with the abundane of transcripts. Inflammation markers have been been taken. The abovementioned cytokines increase in K5-R1/R2 mice as compared to the controls. In both 5M epidermis and 8M dermis, expression of inflammatory cytokines increase by several folds compared to the controls.