Can you please answer the following questions: 1) Small single strand DNA molecu
ID: 213952 • Letter: C
Question
Can you please answer the following questions:
1) Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a
A) primer. B) probe. C) plasmid. D) promoter. E) peptide.
2) The templates for next-generation sequencing are
A) double-strand restriction fragments.
B) double-strand DNA on silicon chips.
C) double-strand PCR products.
D) single-strand DNA on silicon chips.
E) single-strand DNA on slides.
3) Double strand DNA fragments are separated by size using
A) restriction analysis.
B) FISH.
C) PCR.
D) northern blotting.
E) gel electrophoresis.
4) Which of the following statements concerning transcription in bacteria is FALSE?
A) It occurs in the nucleoid region.
B) Sigma factors are parts of RNA polymerase that recognize promoter regions.
C) Different RNA polymerases are required for synthesis of mRNA, rRNA, and tRNA.
D) Termination is either self-induced or due to the presence of Rho protein.
E) There are a variety of sigma factors that affect transcription.
5) The first antimicrobial widely available for treatment of bacterial infections was a synthetic compound which
A) was an antimetabolic analog.
B) was a nucleotide analog.
C) was an attachment antagonist.
D) disrupted cytoplasmic membranes.
E) interfered with bacterial cell wall synthesis.
6) Standard pasteurization methods are used to
A) reduce microbes that cause spoilage.
B) remove all thermoduric microbes.
C) reduce microbes that cause disease.
D) remove all microbes.
E) reduce microbes that cause disease or spoilage.
7) Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?
A) microarray
B) cloning
C) PCR
D) genome mapping
E) microarray or PCR
8) A "zone of inhibition" is associated with which of the following tests used to determine the efficacy of antibiotics?
A) Etest
B) diffusion susceptibility test
C) broth dilution test
D) both the Etest and diffusion susceptibility test
E) both the broth dilution and the MBC tests
Explanation / Answer
1) the correct option is option (b) probe it is small DNA molecules to which the flourophore is attached which specifically goes and attaches to the complementary DNA segment of our desired choice hence when attaches it flourosnce..
3) the correct option is option (e) gel electrophoresis which basically separates the dsDNA on the basis of size..
4) the correct option is option (c) different RNA polymerase is present in eukaryotic organism.. In prokaryotic organism one RNA polymerase can synthesis the RNA..
5) the correct option is option (e) the first was the Penicillin family which basically interfere with the peptidoglycan cell wall formation in the bacteria .!!
7) the correct option is option (c) PCR it helps in the detection of the sequences using the Forward primer and Reverse primer which will only multiply if the desire segment is present..
8) the correct option is option (d) this is the test used for both etest to find the effects of the antibiotics and to find the diffusion suspectible rate..