IDI A Instructor: J. Afmitto Final Exam Advanced Lab Matching For the fellowing
ID: 214289 • Letter: I
Question
IDI A Instructor: J. Afmitto Final Exam Advanced Lab Matching For the fellowing itemes, matich each word with the best description (a-hj a. Membrane filter at one end of a chromatography column b. Outside of a cell e. Inside of a cell d. Treatment made to look gemuine, but it does not contain the product being tested f. A small amount of sample that is collected during cobumn g. A process that uses high frequency sound waves to burst open cells h. The process of releasing molecules from resin during column chromatography 2. Elution 3. Extracellular 4. Fraction 5. Frit 6. Intracellular 7. Placebo & Sonication For the following items, b. Thin-layer chromatography c. Column 9. Resin beads are utilized which have an opposite charge of the protein of interest. A final wash with an elution buffer knocks off the protein of interest and fractions are collected 10. A solvent is used to separate out proteins, deposited on a filter paper, based on their size and solubility 11. Antibody-antigen interactions are the basis of this method. As a sample flows through the antibody-coupled resin, specific epitopes on the protein of interest will bind to the antibodies. 12. Instruments that exert high pressure are attached to sophisticated computers and operated by highly trained technicians to run samples through and collect protein fractions. A glass plate lined with silicia gel is used while a buffer carrying the soluble molecules creeps along the plate, depositing molecules based on their size and solubility in the buffer. 13. For the following items, determine which phase of clinical trials (a-d) best fits each description a. Phase I b. Phase II c. Phase III d. Phase IV 14. Several thousand patients receive treatment with the pharmaceutical. In addition to monitoring safety and efficacy, a comparison with other available treatments is also done.Explanation / Answer
Membrane filter at one end of the chromatography column: Frit
Outside of a cell: Extracellular
Inside of a cell: Intracellular
Treatment made to look genuine, but it does not contain the product being tested: Placebo
Separation of molecules on or through a stationary phase: Chromatography
A small amount of sample that is collected during column chromatography: Fraction
A process that uses high frequency sound waves to burst open cells: Sonication
A process of releasing molecules from resin during column chromatography: Elution