In 1964, Nirenberg and Leder used synthetic mRNA triplets, tRNAs charged with am

Question

In 1964, Nirenberg and Leder used synthetic mRNA triplets, tRNAs charged with amino acids, and ribosomes to determine which amino acid each triplet encoded (see Figure 15.9 in textbook). Where was the correct amino acid found in their experimental system? O. In the filtrate (iquid), attached to an mRNA on a ribosome O On an unbound tRNA in the filtrate (liquid) O On the nitrocellulose filter, attached to an mRNA on a ribosome O On an unbound tRNA captured by the nitrocellulose filter Click Save and Submit to save and submit. Click Save All Ansuers to save all answers Save All Answers Close Wind

Explanation / Answer

In the Nirenberg and Leder experiment, the amino acid was labelled and then the mixture was placed on the filter. The presence of the special fiter allowed the unbound tRNAs to pass through but not the ribosomes that were bound to the amino acid. So, the amino acid is found in the FILTRATE( LIQUID) THAT IS attached to an mRNA on a ribosome.

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