In humans the LCT gene encodes the enzyme lactase which is necessary for metabol

Question

In humans the LCT gene encodes the enzyme lactase which is necessary for metabolizing the disaccharide lactose. Lactase catalyzes the first step in the metabolism of lactose by breaking lactose into the monosaccharides glucose and galactose. Glucose and galactose are then broken down to form ATP by cellular respiration (more on this later in the Carbohydrates Module). Mapa To study LCT gene expression, you sequence a 1 kilobase region surrounding the transcription start site Which of the following would you expect to find? (check all that are correct) AAUAAA for CPSF to bind an A block and a C block for TFIID to bind the -10 and-35 sequences for TFIID to bind binding sites for ancillary transcription factors to bind TATA box to bind TFIID an operator sequence for lac repressor to bind a Many adults become lactose intolerant later in life, which is thought to be due to changes in transcription of the LCT gene. With this hypothesis, you sequence the LCT gene and more of its surrounding genomic sequence in several lactose tolerant and lactose intolerant individuals. You find a number of single basepair mutations only in the lactose intolerant individuals. These mutations are not in the LCT gene, but are located 10-20 kilobases upstream of the gene in the introns of the MCM6 gene (pictured below as bolded nucleotides with slash marks, example- G/A). MCM6 gene LCT gene

Explanation / Answer

for part 1 the marked answers are correct, except the 4th option. There are no other binding sites for ancillary transcription factors. First the transcription factor TFIID (TATA Binding Protein), TFIIB and TFIIF along with RNA polymerase 2 bind to TATA box. Then other transcription factors bind RNA Pol 2 through its Carboxy-terminal domain(CTD).

Part 2 is absolutely correct. MCM6 is an enhancer element.

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