Can you please explain what is going on in this figure. I provided the text that
ID: 217472 • Letter: C
Question
Can you please explain what is going on in this figure. I provided the text that goes with it and the legend. Thank you.
Figure 2. Narrowing down the Candidate Factors (A) Effect of the removal of individual factors from the pool of 24 transduced factors on the formation of G418-resistant colonies. Fbx15bgeo/bgeo MEFs were transduced with the indicated factors and selected with G418 for 10 days (white columns) or 16 days (black columns). (B) Effect of the removal of individual factors from the selected 10 factors on the formation of G418-resistant colonies 16 days after transduction. (C) Effect of the transduction of pools of four, three, and two factors on the formation of G418-resistant colonies 16 days after transduction. (D) Morphologies of iPS-MEF4 (clone 7), iPS-MEF10 (clone 6), and iPS-MEF3 (clone 3). Scale bars = 200 mm.
40 Colony number at day 10 Colony number at day 16 20 0 1 23 4 5 678 9 10 111213 1415 16 17 18 19 2021 2223 24^, /, 24 factors-1 factor 300 200 150 100E 3 factors 2 factors 7 3 4 5 1114 15 18 2021 22701/4 10 factors 1 factor IPS-MEF10-6 iPS-MEF3-3 iPS-MEF4-7Explanation / Answer
Fig 2A shows effect of removal all 24 factors individually on the formation of G-418 resistant colonies for 10 days or 16 days. The white columns are represented for the time period of 10 days and black columns for 16 days.
Fig.2B shows effect of removal of selected 10 factors on the formation of G418 resistant colonies 16 days after transduction.
Fig.2C shows effect of transduction of pools of four, three and two factors on formation of G418 resistant colonies 16 days after transduction.
Fig 2D shows morphology of iPS-MEF4(clone 7), iPS-MEF10 (clone 6), iPS-MEF(clone 3)