INDIVIDUAL ASSIGNMENT: Electripheresis Results-Analy nag DNA Fragments (20 pts)
ID: 219158 • Letter: I
Question
INDIVIDUAL ASSIGNMENT: Electripheresis Results-Analy nag DNA Fragments (20 pts) ttons: Submit Taible 2. Figure 3, and the follow-up questioas (from the results section) to your instructoe IN CLASS at the sart of class next week Failure to hand in all required assegnmen components will result in a reduced grade. Yoa do aot seed so subeeit your work or question cesponses Migration of DNA The size of DNA fragments on an aparose gel can be estimaned by comparnng che bands of snknown sine to the position of bands in a sample containing DNA fragments of krowa size. These DNA s DNA frapmests will be run alonpside your unknown samplkis) in the gel Look at the diagram of an agarose gel (Figure 1). It shous two lanes A lane is the columa below a well and 1,000 bp. The largest fragments are at the top of the gel (closest to the wells) ) are fragments of DNA uhose exact snes are known. Standard Figure I Example gel showing two lanes with DNA bands 2. Label each band in the left lase with its base-pair size Right Lane Actual Distanese 3. Compare the two lanes of bands. Estmate the size of the fragments in the right lane a. Upper band b. Lower band (estimated by eye) (estimated by eye 4 How did you determine the sizes of the tuo bands in the right lane? The number of hase pairs in each of the DNA fragments in the right lane can also be determined using another method that can be more accurate. This involves graphing the size of the known fragments from the DNA standards against the distance esch DNA band curve. This is most conveniently done on semi-log graph paper ed through be gel, so generade a standand 5. Measure the distance in millimeters (mam) that each band in both lases moved in the diagram of the garose gel (Measwe roos the beottoes edge of the well to the boow edge of the bund. Record the informution in Table I below, in the columns labeled "Distance migrated" Make sure you inelude the units of measurement, mm. 133Explanation / Answer
The left lane contains the molecular weight standard because on the basis of the pattern of bands in the gel one come to know about the standard molecular weight in the gel.
In the left lane
In the right lane
One can estimate the band size on the basis of molecular weight standard since the molecular weight standard run pattern is known therefore one can estimate the unknown bandsize in the gel
In the left lane the distance migrated by the band in the gel
In the right lane the distance migrated by the band in the gel