A recombinant DNA was constructed by inserting the DNA of interest into the Amp

Question

A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?

A)no growth on either ampicillin or tetracycline containing media B) growth on ampicillin but not tetracycline containing media C) growth on tetracycline but not ampicillin containing media D) growth only on media containing both antibiotics E) growth on both ampicillin containing media and tetracycline containing media

Explanation / Answer

The following results would be expected:

B)

growth on ampicillin but not tetracycline containing media

As due to insertional inactivation growth will be seen in case of ampicillin but not in case of tetracycline containing media as the TetR gene that is not interrupted.

B)

growth on ampicillin but not tetracycline containing media

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