Please indicate the correct answer. The blue selected answer may or may not be c
ID: 222042 • Letter: P
Question
Please indicate the correct answer. The blue selected answer may or may not be correct
A Ramachandran plot is a useful tool to determine The association state of a protein The isoelectric point of a protein The quality of a protein structure The stability of a protein None of the above Which spectroscopy is most useful for determining protein secondary structure Circular Dichroism spectroscopy Mass spectroscopy NMR spectroscopy UV-Visible spectroscopy of Aromatic residues A and C Disulfide bonds in proteins Can lead to changes in migration on SDS PAGE Can be exchanged in a redox reaction Can be exchanged in an acid base reaction A and B None of the above X-ray crystallography Allows for convenient assessment of the roles of protons in protein function Has resulted in the structures of some of the largest known supramolecular assemblies Is best for acquiring dynamic information about protein structures Provides similar atomic resolutions in most structures Works best for small highly soluble proteinsExplanation / Answer
Ans:
A ramchandran plot is a useful tool to determine:
The quality of a protein structure.
Explanation:
- The Ramchandran plot is usually used in assessing quality of experimental structures or homology models.
- Useful for the same structures refined at different resolutions.
Que 5:
Ans:
Spectroscopy useful for determinig protein secondary structure are:
- Circular Dichorism spectroscopy
- NMR spectroscopy
- Hence the correct option is both A and C.
Explanation:
- CD spectroscopy provides rapid determinations of proteins secondary structure.
- In NMR spectroscopy, chemical shift is used to determine protein secondary structure.