The plasmid pUC18 vector (2.7 kb long) is shown below. What restriction enzymes

Question

The plasmid pUC18 vector (2.7 kb long) is shown below. What restriction enzymes do you use to clone the gene shown below? You must clone the entire gene without breaking it apart. There is only one combination of enzymes. Cloning of a DNA fragment with two different enzymes is very effective. Explain with one sentence. To verify successful cloning of this gene, Dr. Tasked you to digest this recombinant plasmid with PstI. First, draw the recombinant plasmid with HindIII, PstI, and EcoRI recognition sites and distances between them below. (3) Second, draw the pattern of the PstI digest on a gel with fragment sizes.

Explanation / Answer

EcoRI ->Escherichia coli can used as a restriction enzyme as they cut the DNA molecule between hindlll and pstlll

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