What is the experimental basis for concluding that puffs in polytene chromosomes
ID: 223620 • Letter: W
Question
What is the experimental basis for concluding that puffs in polytene chromosomes and loops in lampbrush chromosomes are areas of intense transcription of RNA?
Electron microscopy of RNA-polymerase combined with biochemical analysis have shown a high levels of fluorescence of RNA incorporation, indicating intense transcription.
Radioactively labeled RNA precursors followed by autoradiography have shown a high rate of RNA incorporation, indicating intense transcription.
Fluorescent antibodies against RNA polymerase have shown high levels of fluorescent RNA incorporation, indicating intense transcription.
Density analysis of RNA-polymerase has shown a high rate of RNA incorporation, indicating intense transcription.
a.Electron microscopy of RNA-polymerase combined with biochemical analysis have shown a high levels of fluorescence of RNA incorporation, indicating intense transcription.
b.Radioactively labeled RNA precursors followed by autoradiography have shown a high rate of RNA incorporation, indicating intense transcription.
c.Fluorescent antibodies against RNA polymerase have shown high levels of fluorescent RNA incorporation, indicating intense transcription.
d.Density analysis of RNA-polymerase has shown a high rate of RNA incorporation, indicating intense transcription.
Explanation / Answer
C. Fluorescent antibodies.
Since these tags specifically binds to the RNAP. Electron micrograph showed only the presence of polytene and lampbrush chromosome.