Prepare __ a. cysteine protease b. metalloprotease c. threonine protease d. seri
ID: 225586 • Letter: P
Question
Prepare __ a. cysteine protease b. metalloprotease c. threonine protease d. serine protease e. aspartic protease Match the each following protein tags to their purification mechanism. 'Strep' tag FLAG Inteins a. Use of a column containing Chitin followed by self-cleavage. b. Use of a specific antibody bound to a column or a bead. c. Use of a streptavidin coated beads or column. d. Use of a column containing nickel, followed by imidazole. What is the recombinant protein? new combination of genes that from DNA within living cues. Match each of the recombinant protein product with its function. Factor VIII Interleukin-2 Erythropoietin Somatotropin Tissue plasminogen factor a. promotes red blood cell formation. b. Treats growth hormone deficiency. c. Kills tumor cells. d. Dissolves Wood clots to prevent heart attacks. e. Helps Wood clots form in hemophiliacs. With respect to the following enzymes produced industrially, match each enzyme to its function. Invertase Rennet Catalase Amylase Glucose isomerase Degradation of starch, used in brewing. b. Protease specific to the cheese industry. c. Hydrolysis of sucrose to its monomers d. Conversion of glucose to fructose In high-fructose syrups. e. Antioxidant used in processed foods. What is sited directed mutagenesis for?Explanation / Answer
41) e) aspartic protease
Pepsin is an aspartic protease (breaksdown protein into smaller peptides) as it has one or more catalytic aspartate residues at its active site for cleaving peptide substrates.
42) Strep-tag: c) Use of streptavidin coated beads or column.
Strep-tag is used for the purification and detection of strep-tag fusion proteins in affinity chromatography column with immobilized strep-tactin.
FLAG tag: b) Use of specific antibody bound to a column or a bead.
When there is no antibody for a particular protein the addidtion of a FLAG-tag to a protein allows the protein to be detected by an antibody specific to the FLAG Sequence.
Inteins: a) Use of a column containing chitin followed by self-cleavage.
Affinity tag, Chitin Binding Domains (CBD) is fused with inteins which undergoes self-cleavage reaction at low temperature. Proteins are allowed to run in a column containing chitin which allows CBD to bind to the column and when elution of the protein is required temperature is lowered which cause self-cleavage of the CBD by intein and the protein is eluted.
43) The combination of two or more DNA Sequence (using Rec DNA technology) form multiple source creating sequence which would not otherwise be found in nature is called Recombinant DNA and the proteins which results from the expression of recombinant DNA within living cells is called Recombinant proteins.
44) Factor VIII: e) Factor VIII is present in blood in inactive form until injury occurs which activates Factor VIII which then interacts with Factor IX and initiates blood clot. Factor VII is also known as anti-hemophilic factor.
Erythropoietin: a) it is a hormone controlling the production of red blood cells by acting as a signaling molecules for erythrocytes precursors in the bone marrow.
Somatotropin: b) It is a growth hormone produced by the pituitary gland that stimulates growth and cell reproduction in humans and animals. Growth hormone deficiency can be treated by growth hormone replacement therapy.
Tissue plasminogen factor: d) It is a serine protease which catalyze the conversion of plasminogen to plasmin which is one of the major enzyme for breaking down of blood clots.In medicine it is used to dissolve blood clots to prevent heart attacks.
Interleukin 2: c) It is a cytokine signaling molecules which activatesand regulate immune cells like T-cell and natural killer cell which have the ability to kill cancer cells.