Your PCR product was sequenced by a method known as Cycle Sequencing. Which of t
ID: 226106 • Letter: Y
Question
Your PCR product was sequenced by a method known as Cycle Sequencing. Which of the following statements is false.
A. Tagged terminator nucleotides facilitate the creation of a series of nested DNA sequences of different length.
B. Cycle sequencing is done in a PCR machine.
C. Cycle sequencing can be completed in just one test tube.
DAn automatic sequencer performs electrophoresis and reads the tagged DNA pieces, providing a read out of the nucleotide bases comprising the DNA sequence of the fragment being tested.
Explanation / Answer
In cycle sequencing, four test tubes are required.
One test tube for each ddNTP.
Hence third statement is incorrect.
(C) Cycle sequencing can be completed in just one test tube