QUESTIONS 1. How many polypeptides are present in C3c? Determine their molecular
ID: 227137 • Letter: Q
Question
QUESTIONS 1. How many polypeptides are present in C3c? Determine their molecular masses from the figure 2. What was the purpose of using DTT in this experiment? DDT is used to assist the SDS-PAGE in inducing the protein with a large negative charge so that it will migrate towards the positive pole when placed in an electric field 3. What kind of bonds hold the polypeptides together? Peptide bonds 4. How is the 102 kD polypeptides related to the other polypeptides species? The 102 kD polypeptide contains larger DNA fragments than the 75, 43, and 27 polypeptides. 5. The 43 kD polypeptide shows up as two bands, and a precursor-product relationship appears to apply to them. Interpret this band pattern.Explanation / Answer
Answer:
In the SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) method, the SDS complex acts like a detergent rupturing the tertiary structure of the protein molecules and reducing them to linear molecules. It also masks the charge on the proteins with a uniform negative charge, so that the proteins can be influenced by the electric field proportional to their molecular weights.
As per the figure there are 4 polypeptides present in the C3c having MW of 102 kDa, 75 kDa, 43 kDa, and 27 kDa.