The mechanism of chymotrypsin can be viewed as a two step process?acylation of t
ID: 24271 • Letter: T
Question
The mechanism of chymotrypsin can be viewed as a two step process?acylation of the enzyme active site followed by a deacylation reaction. The observation of "burst" kinetics in rapid kinetic studies of the hydrolysis of N-acetyl-L-phenylalanine p-nitrophenyl ester by chymotrypsin is due to:A. the rate of the acylation reaction being faster than the deacylation reaction.
B. the rates of acylation and deacylation being equal.
C. the rate of the acylation reaction being slower than the deacylation reaction.
Explanation / Answer
A. the rate of the acylation reaction being faster than the deacylation reaction