Assume that you have used PCR and electrophoresis to assay an Alu element polymo
ID: 253792 • Letter: A
Question
Assume that you have used PCR and electrophoresis to assay an Alu element polymorphism at the D1 locus on chromosome 17 in four Asian populations. When the Alu element is not present (-), the DNA fragment is 200 base pairs (bp). When the Alu element is present (+), the DNA fragment is 500 bp.
Compute the heterozygosity (H) for each population. Don’t forget to take into account the sample size of each population! Please show your work, and remember, . [4 points + 2 points for showing your work]
Population
# of individuals
200 bp fragment (-)
500 bp fragment (+)
H
Parsi
110
0.636
0.364
Rajput
104
0.693
0.308
Munda
50
0.69
0.31
Kotas
94
0.76
0.24
Population
# of individuals
200 bp fragment (-)
500 bp fragment (+)
H
Parsi
110
0.636
0.364
Rajput
104
0.693
0.308
Munda
50
0.69
0.31
Kotas
94
0.76
0.24
Explanation / Answer
The proportion of heterozygosity = 2*p*q.
The frequency of heterozgygosity = Number of individuals * proportion of heterozygosity.
Population
# of individuals
200 bp fragment (-) / p
500 bp fragment (+) / q
2pq
H
Parsi
110
0.636
0.364
0.463008
51
Rajput
104
0.693
0.308
0.426888
44
Munda
50
0.69
0.31
0.4278
21
Kotas
94
0.76
0.24
0.3648
34
Population
# of individuals
200 bp fragment (-) / p
500 bp fragment (+) / q
2pq
H
Parsi
110
0.636
0.364
0.463008
51
Rajput
104
0.693
0.308
0.426888
44
Munda
50
0.69
0.31
0.4278
21
Kotas
94
0.76
0.24
0.3648
34