QUESTION 5 You decide that for your next experiment you need to insert a gene in

Question

QUESTION 5 You decide that for your next experiment you need to insert a gene into a plasmid and have the plasmid be taken up by your choice of bacterial species. From the following choices, which would be your first step? Insert the gene of interest into a virus which will serve as a vector 0 Heat shock your bacteria causing them to burst enabling them to take up the plasmid Identify the zone of replication in your plasmid O Either identify the sequence of the gene of interest or the length of the sequence of interest between RE sites

Explanation / Answer

Hello,

While carrying out gene cloning it is very much necessary to obtain a plasmid which could be used to introduce the gene of interest and this is also the very first step to be carried out in the process.

Accordingly we have to first identify the site of DNA replication in the plasmid because this is necessary to replicate inside the host organism.

So the answer here would be to identify the zone of replication in your plasmid.

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