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Microbiology Study Guide 8. The time needed to sterilize an object using moist h

ID: 255863 • Letter: M

Question

Microbiology Study Guide

8. The time needed to sterilize an object using moist heat is the time needed to sterilize to the same level by dry heat. less than B) more than same as 9. All of the following are examples of moist heat methods to kill microbes except A. Boiling at 100°C B. Autoclaving C.Lyophilization D. Pasteurization ltra-high temperature sterilization 10. During an experiment in a microbiology lab, you were using an inoculating loop to introduce a bacterial sample into a sterile broth culture medium. Before picking the bacterial sample, you passed the mouth of the culture tube and inoculating loop through the flame. This method of sterilization is classified as A-Dry heat oist heat C. Radiation D. Desiccation E. Lyophilization 11. Which of the following is approved by Food & Drug Administration (FDA) for sterilization of spices, meats, fruits and vegetables in order to kill contaminating microbes, and eggs and larvae of insects? Dry heat B. Moist heat Radiation D. Desiccation E. Lyophilization 12. Which of the following is a technique used for many years that combines freezing and then drying using a vacuum pump to preserve microbial or cell samples? A. Desiccation B. Autoclaving D. Lyophilization E. Filtration 13. Jams, jellies, pickles and salted fish expose contaminating bacteria to ronment in order to prevent their growth or kill them. ?.juy potonic Isotonic C. Hypertonic 14. Before receiving a vaccine shot, the nurse quickly wipes the area of the skin with an alcohol swab This action mostly A. sterilizes the surface Bo degerms the surface C/disinfects the surface D. is bactericidal

Explanation / Answer

Ans.[1] ? ?higher than [ object using moist heat is more /higher than the time ..............dry heat ]

[2] Boiling 100 degree [?boling at 100 degree for 5-10 min.is susposed to kill non spore forming and few spore forming microorganism.]

?[3] Dry Heat [?sterilized a loop often referred to as flaming a loop is commom componant of aseptic technique.]

?[4] Moist [? boiling is one of the oldest methods ,it is typically quite effective at killing........]

[5] Autoclaving [? sterilization reley on moist heat ]

?[6] Hypertonic [ ?the addition of a solute creat a hypertonic environment ,drawing water out of cell? ]

?[7] Degerming [ to get rid germs .]

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