Can someone please answer the following questions with the right choices Thank y

Question

Can someone please answer the following questions with the right choices Thank you 10. you did a serial 1 in 10 aution of a stock solution of deprenyl. For example, to make a 104 M. solution from a 10. M stock you could take 20 ?1 of the stock and dilute R down with 180 ul of a diluent-the phosphate buffer. How much diluent would you have needed to add to make a 10- M solution from the 10 M stock if you were told to first take 500pal of the stock solution? 4500p b.soooul c. 180ul again d.2Dul 11. In terms of the number of radioactive counts (epm) you would expect to have in each set of tubes in your experiment which of the following options ranks the tubes in terms of owest number of courts to highest number of counts? a.10" M deprenyl > 10 M deprenyl>10 M depreny b control blank > 10 M deprenyl c control 10M deprenyl > 10 M depreny d. 10M deprenyi > 10°M deprenyls Blank e Blank > Control > 10 M depreny 12 what was the highest concentration of deprenyt that you used a. 10M b. 10m M c. 10 M e. 10 M 13, there was a centrifugation step in your essay What was the perpose of this step? a. To stop the assay by separating out the reaction mixture b. Te ensure that all the solutions in the assay were thoroughly mx e To separate out youe reaction mixture so that the solvent layer was on the top and the aqueous layer at the bottom of your tubes. d. To pellet the rat tissue so that it didn't interfere with the assary e to separate out your reaction mixture so that the aqueous layer was on the top and the solvent layer at the bottom of yo ur tubes 14. What was the source of the eneyme used in your assay? a. Homan brain b PEA c Rat brain FocusT

Explanation / Answer

Dilution =1/10

Now 20/180 should equal 500/X, where is the diluent volume.

Upon calculation ,

X=500×180/20

X= 4500ul

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