Can I get help on 46, 6 and 9 Only please !! Thank you! I hope this enough info

Question

Can I get help on 46, 6 and 9 Only please !! Thank you! I hope this enough info to answer the questions..

ah. In the following scheme, write the corret dilution of the original sample for each volume plated and in the space below your dilution answer, write the number of colonies that could theoretically form on each of the corresponding plates (o pts) mL ImL This culture contains 5.2 x 10/ml 99 ml 99 ml 90 ml 99 ml plate 0.1 ml onto plate 'a plate 0.1 ml onto plate 'b plate 0.1 ml onto plate 'c plate 1.0 ml onto plate 'd' plate b plate c plate d plate a lledon plaialiitio aiea dilution plated dilution plated Theoretical number of CFUs that could form on each plate even if above or below the ideal target range

Explanation / Answer

46) Final volume = 99+1 = 100ml

Dilution = 1/100 = 10-2

Dilution for each tubes = 10-2, 10-4, 10-6, 10-7

CFU = number of colonies / (amount plated*dilution)

CFU for plate a = 5.2 *109/ 0.1* 10-2 = 52 * 1011

CFU for plate b = 5.2 *109/ 0.1* 10-4 = 52 * 1013

CFU for plate c = 5.2 *109/ 0.1* 10-6 = 52 * 1015

CFU for plate d = 5.2 *109/ 0.1* 10-7 = 52 * 1016

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