Monooxygenases are able to incorporate one atom from molecular oxygen into the s

Question

Monooxygenases are able to incorporate one atom from molecular oxygen into the substrate while converting the other oxygen into water. In the first step of the catalytic cycle, the cofactor undergoes a 2-electron reduction supposedly via hydride ion transfer from NAD(P)H. The reduced flavin reacts rapidly with molecular oxygen to form a peroxyflavin that is stabilized by the bound NAD(P). After a suitable substrate enters the active site of the enzyme, the flavin peroxide attacks the electrophilic carbonyl of the substrate, resulting in one atom of molecular oxygen being transferred to the substrate with the other forming water reduced flavin X,H' NADIP)H xo ?? oxidized flavin NADIP), HO 1. How many electrons the reduced flavin has? B. 2 C. 3 D. 4 2. What are FAD and NAD(P)H relationships to this enzyme? A. FAD is cofactor; NAD(P)H is coenzyme B. FAD is coenzyme; NAD(P)H is cofactor C. FAD is cofactor; NAD(P)H is prosthetic D. FAD is prosthetic; NAD(P)H is cofactor

Explanation / Answer

The reduced flavin has 2 electrons.

FAD is prosthetic group and NAD(P)H is a cofactor.

Flavin adenine dinucleotide is a redox cofactor, more specifically a prosthetic group of a protein.

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